Synthetize polyhydroxyalkanoates using Scenedesmus acuminatus, as an alternative for oil derivated plastics.

Abstract

The indiscriminate use of plastic causes environmental problems; is estimated that around 500 year are required for its total degradation. The strategies for its recycle require the technology development and the users’ conscience.  The alternatives which are made by biotechnological methods are not sustainable, due to the elevated cost compared with oil derivate. The polihidroxialcanoatos (PHA) are polyesters classified on function of the length of the acid fats chain and synthetized by microorganism with proprieties similar to the polypropylene. The present study objective is to evaluate the potential of the Scenedesmus acuminatus strains on the PHA production. A growing kinetics on 30 test tubes with 20 ml as Bold cultivate mean and 5 ml of strain. We monitored it a 72 hla frequency of extracellular acid fats production and cellular biomass. The acid fats presences were determinate with black sudan ink and composed microscopy at 40 X objective.  From the day fifth teen on acid fats on the cellular supernatant can be seen. The acid fats were proportional to the incubation days and according to the PHA literature they are produced on cellular stress, when there is a lack of macronutrients in cultivate and high quantity of carbon. Scenedesmus has the capacity to use the CO₂ as a carbon source, which reduce the operation cost.